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Table 2 The expression of RBCS1 isoforms in leaves of different coffee genotypes.

From: RBCS1 expression in coffee: Coffea orthologs, Coffea arabica homeologs, and expression variability between genotypes and under drought stress

Genotype

Cultivar

Origin

Trial

CaCc/CaCe

C. canephora

 

L21

 

I

65.93

 

14 T

Conilon

G

1324.28

 

22 S

Conilon

G

247,10

 

73 T

Conilon

G

260.65

 

120 T

Conilon

G

236.71

C. arabica ("pure")

 

Rubi S

Mundo Novo x Catuaí

E

0.00013

 

Bourbon

 

I

0.00014

 

Typica

 

I

0.00017

 

Catuaí

Mundo Novo x Caturra

I

0.00021

C. arabica ("introgressed")

 

HT832/1

Timor hybrid

E

0.00004

 

HT832/2

Timor hybrid

I

0.40102

 

Icatú

C. canephora x Bourbon

I

9.33

 

IAPAR59 T

Villa Sarchi x HT832/2 (Sarchimor)

I

3.22

 

Tupi

Villa Sarchi x HT832/2 (Sarchimor)

I

2.63

 

Obabã

[Villa Sarchi x HT832/2] x Catuaí

I

1.26

 

IPR97

Sarchimor

I

4.98

 

IPR98

Sarchimor

I

21.65

 

IPR102

Icatú x Catuaí

I

0.00427

 

IPR106

Icatú x Catuaí

E

0.03212

 

IPR107

Sarchimor x Mundo Novo

E

0.12255

C. eugenioides

  

I

0.00035

  1. Expression was measured by the ratio CaCc/CaCe where CaCc (RBCS1-Cc) and CaCe (RBCS1-Ce) values were obtained using the C18244 and E18244 primer pairs (Table 1), respectively. Relative quantifications (RQ) were normalised using the expression of the CcUBQ10 (in the case of C. canephora) or GAPDH (for other species) reference genes. The CaCc/CaCe ratio corresponded to (1+E)-ΔCt, where ΔCt = CtmeanCaCc - CtmeanCaCe with E as the efficiency of the gene amplification. Leaves were collected from plants grown in the field at the Embrapa Cerrados (E), IAPAR station (I) and UFV greenhouse (G). When known, the reaction to drought is indicated (T = Tolerant and S = Susceptible). All Sarchimors are derived from HT832/2.
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BMC Plant Biology

ISSN: 1471-2229

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