Figure 1

Alignment of coffee RBCS1 nucleic sequences. Sequences of the CaRBCS1 cDNA [58] from C. arabica cv. Caturra (a) [Genbank:AJ419826] and of the corresponding gene (b) [GenBank:AJ419827] without introns, were aligned with the unigenes SGN-U607188, SGN-U607190 and RBCS1-Cc (identical to SGN-U617577 formed by the alignment of 145 reads found in leaf cDNA libraries from C. canephora) from the SOL genomic database [56] and with the unique RBCS1 homologous read of C. eugenioides [4]. The SGN-U607188 and SGN-U607190 unigenes were formed by the alignment of reads found in cDNA libraries from fruits and the leaves of C. arabica plants. The coding sequences of the partial RBCS1 genes from genotypes of C. arabica [Genbank:DQ300266 to DQ300277; L.S. Ramirez, unpublished results] that matched with CaRBCS1 sequences are underlined in grey, while base differences are boxed in black. The CcRBCS1 cDNA sequence [GenBank:FR728242, this work] corresponded to the underlined sequence of the SGN-U617577 unigene. For all the sequences, the coding sequence is in uppercase, and the 5' and 3' UTR regions are in lower case. Horizontal arrows as well as nucleotides in bold and italics indicate the primers (Table 1) used for qPCR reactions. The stars below the alignments indicate identical bases, and the nucleotides are numbered for each lane.