Starch granules at the bottom of Eppenforf tube pelleted during thylakoid preparation. For each of thylakoid isolation, 1 g of wild type (WT) or transgenic HC-Pro (HC-Pro) leaves (fresh weight, FW) was used. Three biological replicates are presented in the figure. The amount of starch was also quantified after removing the soluble sugars (on the right). The quantification of starch indicated about four-times less starch in HC-Pro expressing leaf samples than in wild type tobacco leaf samples (n = 4).