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Figure 2 | BMC Plant Biology

Figure 2

From: A cytochrome P450 monooxygenase commonly used for negative selection in transgenic plants causes growth anomalies by disrupting brassinosteroid signaling

Figure 2

Relationships between aberrant growths, represented as plant height, and AtSUC2 and P450 SU1 transcript abundance. (A) OCP, WT, cSUC2, and uidA lines arranged by phenotype severity, with plant height of the indicated lines at full maturity (i.e., senescent and ready for seed harvesting), n = 6, variation is expressed as standard deviation. (B) Semi-quantitative RT-PCR of P450 SU1 (black bars) and AtSUC2 (white bars) transcript levels relative to UBQ10 transcript, encoding ubiquitin, n = 3, variation is expressed as standard deviation. (C) Representative gel used to calculate transcript abundance. See Materials and Methods for details.

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