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Figure 2 | BMC Plant Biology

Figure 2

From: Reactive oxygen species and transcript analysis upon excess light treatment in wild-type Arabidopsis thaliana vs a photosensitive mutant lacking zeaxanthin and lutein

Figure 2

Steady-state accumulation of ROS species and protein oxidizing activity in wild type and npq1lut2 mutant plants. Specific probes were used to quantify the accumulation of several ROS in wild type and npq1lut2 detached leaves under stress (1000 μmol photons m-2 s-1, 10°C). (A) DFC and (B) ProxF fluorescence was used to follow the accumulation of reduced forms of ROS. (C) SOSG fluorescence was used to follow singlet oxygen (1O2). Details on ROS measurements are given in material and method session. Symbols and error bars show means ± SD. (D) Western-blots were used to detect oxidized thylakoid proteins extracted from wild type and npq1lut2 membranes. WT and npq1lut2 rosettes were pre-treated for 48 h at 10°C and low light as described in methods, then were exposed to photoxidative conditions (1000 μmol photon m-2 s-1, 10°C, 16 h light/8 h dark). Leaves were harvested and thylakoids isolated before stress (0) and at same time after 1, 2 and 5 days of HL.

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BMC Plant Biology

ISSN: 1471-2229

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