Figure 1

Characterization of AtrabD2b and AtrabD2c mutations. A, Gene map. The scaled linear map depicts the 8 exons as boxes and the 7 introns as lines between the boxes for both the AtRabD2b and AtRabD2c genes. The positions of the translational start and stop codons in exon 1 and exon 8, respectively, are noted. The locations of the T-DNA insertions (not drawn to scale) in the genes are indicated. B, Genotypes of T-DNA insertion mutants. Genomic DNA was isolated from the indicated single and double mutants and amplified by PCR. Primer pairs used were as following: lane 1, AtrabD2c-LP1 and AtrabD2c-RP1; lane 2, AtrabD2c-RP1 and LBb1; lane 3, AtrabD2b-LP1 and AtrabD2b-RP1; lane 4, AtrabD2b-RP1 and LBb1. C, Analysis of transcripts from AtrabD2b-1, AtrabD2c-1 and AtrabD2b/2c mutants. Total RNA from leaves of wild-type plants, AtrabD2c-1, AtrabD2b-1 and AtrabD2b/2c was amplified by RT-PCR. Primer pairs for AtRabD2c were AtRabD2c-F and AtRabD2c-R, primer pairs for AtRabD2b were AtRabD2b-F and AtRabD2b-R. Tubulin was used as control.