Figure 3

Chemically inducible complementation of the pf14 mutant by the CYC6:RSP3-HA construct. Percentage of swimming (S) and flagellated-immotile (F/I) cells of two transformants, 48 hours after Ni addition. The transformants were grown in TAP ENEA2 medium in 24-well microtiter plates and induced at mid-log phase with 25 μM Ni. For details, see Methods.