Figure 2
From: Conservation of ciliary proteins in plants with no cilia
A) Phylogenetic distribution of the filtered conserved in ciliated species and non-ciliated land plants, but absent in other non-ciliated species (CCP) proteins from the reciprocal best BLAST analysis. Ciliated species are shown in blue and non-ciliated land plants in green. B) Western blot of whole cell lysates with anti-TY antibody (above) and loading control anti-PFR2 antibody (below). C) Direct fluorescence of whole cell Trypanosoma brucei showing cellular localisation of TbCCP1 (Tb11.02.1370), TbBUG22 (Tb10.70.5560) and TbCCP2 (Tb927.8.5380). Left panel showing fluorescence signal from YFP and an overlay in right panel of signal from YFP (green), DAPI (magenta) and phase-contrast. All three possess a ciliary localisation (white arrows point to flagella (CCP1 and BUG22) and basal bodies (CCP2)). D) Direct fluorescence of whole cell Trypanosoma brucei showing cellular localisation of TbCCP2 (Tb927.8.5380). Expression is cell-cycle regulated (white arrows depict basal bodies). Scale bar = 2 μm.