Figure 2

NFS1, NFS2 and ISD11 gene expression in root and leaf. Quantitative RT-PCR analysis of (a) NFS1, (b) NFS2 and (c) ISD11 gene expression in soybean tissues from total root and leaf RNA. Relative expression level was measured by performing PCR in four biological replicates and four technical replicates for each biological replicate per tissue with SE shown. Values were normalized against F-BOX and MET. a and b indicate difference between tissues for each gene. 1 and 2 indicate difference between genes in each tissue. * indicates difference in sum.