Figure 5
From: High resolution melting analysis for the detection of EMS induced mutations in wheat Sbella genes
Semiquantitative and quantitative RT-PCR of SBEIIa transcripts. a) Semiquantitative RT-PCR of SBEIIa genes in SBEIIa homozygous single mutant genotypes: 1) SBEIIa-A-1; 2) SBEIIa-A-2; 3) SBEIIa-D-1; 4) SBEIIa-B-1; 5) SBEIIa-B-2; 6) wild-type Cadenza. b) Relative expression of SBEIIa homoeologs in single null genotypes as determined by Real Time quantitative PCR analysis: W.T.= wild type Cadenza; A-(1)= SBEIIa-A-1; A-(2)= SBEIIa-A-2; B-(1)= SBEIIa-B-1; B-(2)= SBEIIa-B-2; D-(1)= SBEIIa-D-1. Vertical bars indicate standard error.