Gene expression analysis of γ-TMT1 , γ-TMT2 , and γ-TMT3 in leaves of Ichihime and KAS. Quantitative RT-PCR was performed on total RNA from leaves using the gene-specific primers listed in Table 5. Yellow bars represent Ichihime and blue bars represent KAS. Transcript levels were normalized with the values obtained for the internal control (β-tubulin mRNA). Values represent the mean of three replicates ± SD. Asterisks show significant difference between the Ichihime genotype group and the KAS genotype group (confidence interval 95%).