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Table 2 Proteolytic activity of purified PvNod41

From: Nodulin 41, a novel late nodulin of common bean with peptidase activity

Inhibitor

Concentration

% of residual activity (n = 3)

Pepstatin A

2 μM

55 (± 5)

2-mercaptoethanol

25 mM

62 (± 5)

Fe3+

10 mM

39 (± 4)

SDS

0.05%

47 (± 5)

EDTA

5 mM

100

  1. Purified PvNod41 was tested for activity using casein as a substrate in 50 mM sodium citrate, pH 4.5 at 37°C. The enzyme was preincubated in the presence of the indicated inhibitor for 15 min at 37°C before adding the substrate.
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BMC Plant Biology

ISSN: 1471-2229

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