Figure 4
From: Nodulin 41, a novel late nodulin of common bean with peptidase activity
Preferential binding of PvNod41 to denatured proteins. (A) PvNod41 binding assay. Purified PvNod41 was incubated with either native (N) or denatured (D) proteins pre-immobilized on agarose-beads. After incubation, samples were extensively washed with PBS. PvNod41 that was bound to immobilized proteins on the matrix was recovered by boiling the sample with Laemmli buffer and analyzed by 12% SDS-PAGE and Coomassie Brilliant Blue staining. BSA, Bovine Serum Albumin; α2M, α2-Macroglobulin. (B) Far western blot assay. Bovine serum albumin (BSA) and casein, either native or denatured by boiling were blotted onto nitrocellulose, probed with purified PvNod41, and immunodetected with anti-PvNod41 antiserum as described in the Methods section.