Figure 2

GmNAC6 promotes cell death in planta. A. Three-week-old tobacco leaves were infiltrated with Agrobacterium cells carrying the 35S::YFP-NAC6 construct or an unrelated 35S::NIG construct. A. The yellowing phenotypes and necrotic lesions caused by GmNAC6 expression. Leaf sectors were infiltrated with the indicated Agro-inoculum and photographs were taken at 5 days (5 d), 6 days (6 d) and 7 days (7 d) post-Agro-inoculation. B. Transient expression of NIG and GmNAC6 genes in Agro-infiltrated leaf sectors at 5 days after Agroinfiltration. Semi-quantitative RT-PCR was on RNA of Agro-infiltrated leaf sectors with gene-specific primers, as indicated in the figure. C. Chlorophyll loss in the 35S::GmNAC6-infiltrated sectors. Total chlorophyll was determined from the leaf sectors Agro-infiltrated for 5 days with the samples in (A). The values are given as mean ± SD from three replicates. D. Lipid peroxidation induced by GmNAC6 expression. The lipid peroxidation in the 5-d-infiltrated leaf sectors from (A) was monitored by determining the level of TBA-reactive compounds. The values are given as mean ± SD from three replicates. Asterisks indicate values significantly different from the control treatment (p < 0.05, Tukey HSD test). E. The induction of the senescence-associated gene, NTCP23, and pathogenesis-related gene 1, PR1, by GmNAC6 expression. Total RNA was isolated from 5-day-infiltrated leaf sectors that were infiltrated with 35S::GmNAC6 (gray bars) or 35S::GmNAC1 (white bars), and the gene induction was monitored by quantitative RT-PCR using gene-specific primers. Values are relative to the control treatment (NIG infiltration) and asterisks indicate statistic differences (p < 0.05, Tukey HSD test).