Caleosin expression pattern during olive anther development. (A) Coomassie-stained gel of total proteins from olive anthers at the pollen mother cell (PMC), tetrad (Te), microspore (Mi), young pollen grain (YP), mid bicellular pollen grain (MBP) and mature pollen grain (MP) stages. (B) Western blot as in figure 2A probed with a FL anti-Clo3 Ab, followed by an anti-rabbit IgG Alexa 633-conjugated secondary Ab. A band of about 30 kDa (arrow) was recognized by the FL Ab. (C) Densitometric data corresponding to the 30 kDa band from figure 2B.