Table 2 Overview of experiments and microscopic methods
From: Fusarium graminearumforms mycotoxin producing infection structures on wheat
Microscopy of infection | Detached in vitroassay | Intact in vivoassay |
|---|---|---|
Cultivar/strain combinations (Figure no.) | Nandu/WT (1) Nandu/TRI5prom::GFP (2) Nandu/ΔTRI5-GFP (4) Sumai 3/TRI5prom::GFP (2*) | Nandu/TRI5prom::GFP (3*) Amaretto/TRI5prom::GFP (3*) |
Time points of MZFLIII screening | all cultivar/strain combinations 6 hpi - 14 dpi (1 A, 2 A-C, 4 A-C, 2 A-C*, 3 A-F*) | 6 hpi - 7 dpi (3 A-F*) |
Microscopy with Axio Imager Z1 | all cultivar/strain combinations (2* D) | Nandu/TRI5prom::GFP Amaretto/TRI5prom::GFP (3 G*) |
CLSM | Nandu/TRI5prom::GFP (2 D, 1*) | - |
SEM | Nandu/TRI5prom::GFP (2 E-F) Nandu/ΔTRI5-GFP (4 D-F) | - |