Figure 4

Expression of tetrapyrrole biosynthesis genes in tspo-1 mutant. qRT-PCR analyses of tetrapyrrole biosynthesis genes in Col-0, tspo-1 and gun5 5-days-old seedlings grown in constant light. Relative amounts were calculated and normalized relative to Col-0 non-treated (100%). With the exception of HEMA2 and FC1, all the genes have been show to be regulated by light. The data are presented following the enzymes order in the tetrapyrrole biosynthesis. The ACTIN and 18S rRNA genes were used as control. ACTIN, At3g18780; 18S rRNA, At3g41768; (A) HEMA1 (Glutamyl-tRNA reductase 1 - At1g58290) (B) HEMA2 (Glutamyl-tRNA reductase 2 - At1g04490); (C) PPO (Protoporphyrinogen oxidase - At4g01690); (D) FC1 (Ferrochelatase 1 - At5g26030); (E) FC2 (Ferrochelatase 2 - At2g30390); (F) GUN2 (Heme oxygenase 1 - At2g26670); (G) GUN4 (Regulator of Mg-porphyrin synthesis - At3g59400); (H) GUN5 (Mg-chelatase subunit H - At5g13630); (I) CAO (Chlorophyllide A oxygenase - At1g44446); and (J) GUN1 (Pentatricopeptide repeat (PPR) protein - At2g31400). Data shown represent mean values obtained from independent amplification reactions (n = 3) and biological replicates (n = 2). Relative expression levels were calculated. Bars represent the standard error of biological replicates.