Production of viable male unreduced gametes in Brassica interspecific hybrids is genotype specific and stimulated by cold temperatures

Table 2 Genetic identity in an experimental interspecific hybrid plant population.

Species in cross	Genotype ♀ × ♂	No. plants total	True hybrids from molecular marker results, but with abnormal phenotype	Matromorphs (failed hybridity test, maternal phenotype)	True hybrids from molecular marker results and phenotype	Genotype ♀ × ♂	No. plants total	True hybrids from molecular marker results, but with abnormal phenotype	Matromorphs (failed hybridity test, maternal phenotype)	True hybrids from molecular marker results and phenotype
B.
juncea	J1 × C1	15	1^a	0	14	C1 × J1	1	0	1	0
&B.	J1 × C2	6	0	0	6
carinata
B.	J1 × N1	3	0	0	3	N1 × J1	9	0	0	9
juncea	J1 × N2	3	0	0	3	N2 × J1	3	1^b	0	2
&B.	J1 × N3	3	0	0	3	N3 × J1	1	0	0	1
napus	J1 × N4	3	0	0	3	N4 × J1	1	0	1	0
	J1 × N5	7	0	0	7	N5 × J1	4	3^b	0	1
	N1 × C1	5	0	0	5	C1 × N1	1	0	0	1
B.	N1 × C2	5	0	0	5	C2 × N1	1	0	0	1
napus	N2 × C2	3	0	0	3	C2 × N2	3	0	0	3
&B.	N3 × C1	1	0	0	1
carinata	N4 × C1	6	0	4	2
	N4 × C2	5	0	0	5	C2 × N4	1	0	0	1
	Total	65	1	4	60		25	4	2	19

^a missing some marker loci from B. carinata parent, presumed aneuploid gamete
^b Two copies of alleles from female parent to one copy of alleles from male parent, presumed unreduced female gamete.
Hybridity was confirmed using molecular marker analysis and phenotyping. True hybrids from molecular marker results which had abnormal phenotypes were further characterized using ten additional dosage sensitive molecular markers. B. juncea genotype "J1", B. napus genotypes "N1", "N2", "N3", "N4" and "N5" and B. carinata genotypes "C1" and "C2" were crossed to produce the experimental hybrid population, and a subset of the seeds produced sown out.

ISSN: 1471-2229