Figure 4

Nulli-Tetra lines for the group 3 chromosomes in Chinese Spring genetic stock lines analysed for occurrence of TaEXPB11cs1 (left panel) and TaEXPB11cs2 (right panel). A (left panel). For the chromosome assignment of TaEXPB11cs1, PCR products assaying exon 1 with one of the primers located in the insertion that characterizes TaEXPB11cs1 (see Material and Methods) were analysed on 2% agarose gels and stained with SYBR Green. The molecular markers are a 100 bp ladder (Axygen) and the product size of just over 1000 bp was as expected from the genome sequence (1027 bp). B (right panel). For the chromosome assignment of TaEXPB11cs2 (right panel), SNPs in exon 1 that differentiated the genes on chromosome 3A, 3B and 3D were assayed by direct sequencing of PCR products from primers that amplified a common section of this exon. All possible Nulli-Tetra combinations were assayed and the sequences compared to the respective sequence from the TaEXPB11wy, TaEXPB11hp and TaEXPB11cs2 genes (top of figure) in order to assign SNPs to particular chromosomes in the Nulli-Tetra combinations.