Epigenetic chromatin modifiers in barley: IV. The study of barley Polycomb group (PcG) genes during seed development and in response to external ABA

Table 2 Primers used for expression analysis and mapping.

Gene	Primer	Sequence (5'-3')	Tm (°C)	Fragment (bp)
	Expression
HvFIE	HvFIE F1	GTGCCCGATTGTGACATTTGGTTT	70	264
	HvFIE R1	CACGGCGGTTTTTGCTTCCTTC	68
HvE(Z)	HvE(Z) F1	AAGCATCTGGTTCTCATCGTC	62	293
	HvE(Z) R1	TGGTCCACCTCAACAAGTAGA	62
HvSu(z)12a	HvSu(z)12a F1	TCGTGACTCGGTAGGAAACATTG	68	261
	HvSu(z)12a R1	CCAATGTTAATAGGTGTTTCAAG	62
HvSu(z)12b	HvSu(z)12b F1	GTATATGAGTTGAGCATAGTGC	62	278
	HvSu(z)12b R1	TTCAGATGGATTTCATTACAGC	60
HvSu(z)12c	HvSu(z)12c F	ATGTGCCGTCAACCGTCCACG	68	463
	HvSu(z)12c R	CTCCAGCTTGCCCTTGTGAAG	66
HvActin	Barley Actin F	ATGGCTGACGGTGAGGACATCCAGCC	72	1111
	Barley Actin R	TCAGAAGCACTTCCTGTGGACGATCGC	71
	HvActin F	GCCGTGCTTTCCCTCTATG	60	235
	HvActin R	GCTTCTCCTTGATGTCCCTTA	62
HVA22	HVA22 F	TGGCGCTCCCGCAGTTCAAG	64	123
	HVA22R	CCTTGAGTATGTGCACCTTGTG	62
	Mapping
HvFIE	HvFIE F2	GTGGGAGCCAAAAACAAAAG	60	262
	HvFIE R2	GGCGTCACAGGCTAAGTAAGG	61
HvE(Z)	HvE(Z) F2	AAATCAGATGTTGCAGGATGG	60	550
	HvE(Z) R2	GCTCCCTTGCATAGATACCAAC	61
HvSu(z)12a	HvSu(z)12a F2	ACTCGTGCAGAACCCAAGAC	60	576
	HvSu(z)12a R2	ACCCATCCCAGCTACAACAG	60
HvSu(z)12b	HvSu(z)12b F2	ATATTCCTTGGGCCTGTGAG	59	308
	HvSu(z)12b R2	TGGAACAGAACACGACGAAG	54

ISSN: 1471-2229