Table 2 Description of candidate reference genes, primers and amplicons for flax housekeeping gene selection.

ACT

Actin

5'-TCCAGGCCGTTCTTTCTCTA-3'

153

97.9

5'-CTGTAAGGTCACGACCAGCA-3'

CYC

Cyclophilin

5'-TGATTGCGGTCAGCTGTAAG-3'

147

93.5

5'-AGGTGAAACGCTAGGCAGAA-3'

EF1A

Elongation Factor 1-α

5'-GCTGCCAACTTCACATCTCA-3'

140

101.9

5'-GATCGCCTGTCAATCTTGGT-3'

EF2

Elongation Factor 2

5'-GTGGTGCTGAGATCACGAAA-3'

109

98.9

5'-AGACGGTTATGCTTGTTGGG-3'

ETIF1

Eukaryotic translation initiation Factor 1

5'-CCTTGTAGGGCTGAGGGATT-3'

145

104.8

5'-CTCATCAAGACCACCAGCAA-3'

ETIF3E

Eukaryotic translation initiation Factor 3 E

5'-TTACTGTCGCATCCATCAGC-3'

106

101.8

5'-GGAGTTGCGGATGAGGTTTA-3'

ETIF3H

Eukaryotic translation initiation Factor 3 H

5'-CAGCGTGCTTGAAGTAACCA-3'

115

101.7

5'-AACCTCCCTCAAGCATCTCA-3'

ETIF4E

Eukaryotic translation initiation Factor 4 E

5'-TGTGGCTCGAAACTCTGATG-3'

145

103.5

5'-CCCATCTGGACAGCTTCATT-3'

ETIF5A

Eukaryotic translation initiation Factor 5 A

5'-TGCCACATGTGAACCGTACT-3'

159

103.1

5'-CTTTACCCTCAGCAAATCCG-3'

GAPDH

Glyceraldehyde 3-phosphate dehydrogenase

5'-AGGTTCTTCCCGCTCTCAAT-3'

138

101.7

5'-CCTCCTTGATAGCAGCCTTG-3'

UBI

Ubiquitin

5'-CTCCGTGGAGGTATGCAGAT-3'

114

94.7

5'-TTCCTTGTCCTGGATCTTCG-3'

UBI2

Ubiquitin extension protein

5'-CCAAGATCCAGGACAAGGAA-3'

129

98.6

5'-GAACCAGGTGGAGAGTCGAT-3'

TUA

α-Tubulin

5'-CCTGTTGGGAGCTTTACTGC-3'

100

102.6

5'-AAGGTGTTGAAGGCATCGTC-3'