Figure 8

Production of ROS and the stress tolerance of WT and the DnaJ mutant j8 , j11 and j20. A, Histochemical detection of H₂O₂ in the leaves with DAB staining after 6 h incubation of leaves under GL (120 μmol photons m^-2 s^-1) and HL (1000 μmol photons m^-2 s^-1). B, Immunoblots depicting the levels of H₂O₂-detoxifying enzymes in WT and the DnaJ mutant leaves after 6 h incubation of plants under different light conditions. 10 μg of the leaf total proteins loaded. C, Ion leakage induced by 6 h HL (1000 μmol photons m^-2 s^-1) illumination of leaves in the presence and absence of Methyl viologen (MV), the values are means ± SD (n = 8) of two independent experiments with 4 replicates. D, OxyBlot of leaf total proteins (10 μg proteins loaded) after treatment of plants at different light intensities. GL, 120 μmol photons m^-2 s^-1 growth light; HL-1, 500 μmol photons m^-2 s^-1 high light; HL-2, 1000 μmol photons m^-2 s^-1 high light. WT, wild-type.