Figure 2

Phenotypic evaluation of NILs. (A) Different stages of the developing soybean seed are shown. Stages one to four correspond to the seed fill stages that were harvested for phenotypic evaluation and concurrently used for gene expression profiling in this study. Stage 1 = 25 to 50 mg seed. Stage 2 = >50 to 100 mg seed. Stage 3 = >100 to 200 mg seed. Stage 4 = >200 to 300 mg seed. Shown in the diagram are 25 mg, 50 mg, 100 mg, and 200 mg seed sizes. (B) Crude protein profiles graphed on a w/w% dry matter basis for the different stages of developing seed (stages one to four) and the final mature soybean seed. Protein profiles are graphed for both the low protein line (LoPro) and the high protein line (HiPro). (C) Crude oil profiles graphed on a w/w% dry matter basis for the different stages of developing seed (stages one to four) and the final mature soybean seed.