Figure 2

LM detection of H ₂ O ₂ with TMB at different developmental stages of the olive flower. A: the presence of H₂O₂ is shown by a dark purple precipitate appearing shortly (c 15 minutes) after the incubation with the appropriate medium (black arrows). This precipitate is clearly distinguishable from the dark brown colour appearing at the latest stages of flower development (white arrows). The last row of pictures shows some details of the labelling at larger magnification. B: quantification of the labelling intensity detected over the stigma surface. C: quantification of the labelling intensity over the anther surface. Both the average and the standard deviation displayed in the graphs correspond to the measurement of a minimum of nine images, on three independent experiments. A: anther; AU: arbitrary units; O: ovary; P: petal; S: stigma.