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Table 3 PCR amplification conditions

From: Sequence analysis of two alleles reveals that intra-and intergenic recombination played a role in the evolution of the radish fertility restorer (Rfo)

Primer pair
(upper primer lower primer)
purpose Annealing temperature Extension time
F24D7-9radR
F24D7-9radF
Screening of BAC library 55.5°C 1 min
F24D7-13radR
F24D7-13radF
Screening of BAC library 54°C 1 min
PPRA:20505U22
PPRA:20954L21
RT-PCR on PPR-A and PPR-2 52°C 30 sec
Rfocons1047U22
PPRB:13225L22
RT-PCR on PPR-B 52°C 1 min 15 sec
PPR1:21229U22
PPRB:13225L22
RT-PCR on PPR-1 51°C 1 min