Primer pair (upper primer lower primer) | purpose | Annealing temperature | Extension time |
---|---|---|---|
F24D7-9radR F24D7-9radF | Screening of BAC library | 55.5°C | 1 min |
F24D7-13radR F24D7-13radF | Screening of BAC library | 54°C | 1 min |
PPRA:20505U22 PPRA:20954L21 | RT-PCR on PPR-A and PPR-2 | 52°C | 30 sec |
Rfocons1047U22 PPRB:13225L22 | RT-PCR on PPR-B | 52°C | 1 min 15 sec |
PPR1:21229U22 PPRB:13225L22 | RT-PCR on PPR-1 | 51°C | 1 min |