Figure 1

Accession CGN0524 harbors a natural candidate mlo allele. A. Southern blot analysis of natural mlo candidate accessions. Genomic DNA of indicated barley accessions was digested with either Eco RV or Hin dIII, blotted onto a nylon membrane and probed with a radiolabelled full-length Mlo cDNA fragment. White arrowheads indicate fragments of the wild-type Mlo copy, black arrowheads point to the prominent mlo-11-characteristic signals (see also [11]). Approximate sizes of these fragments were calculated based on the DNA sequence of the Mlo genomic locus [42] and the arrangement of the mlo-11-specific repeats [11]. B. Single cell complementation of accession CGN0524 by transient gene expression. Leaves of CGN0524 were either bombarded with a plasmid encoding the GUS reporter protein or co-bombarded with the GUS reporter plasmid and a plasmid harboring the wild type Mlo cDNA. Fungal entry rates in transformed (GUS-stained) cells were scored at 48 hours post inoculation. Results show the mean ± standard deviation of n = 3 experiments for expression of GUS alone and n = 5 experiments for expression of GUS + Mlo. The asterisk indicates a statistically significant difference (p < 0.01) from the GUS control according to Student's t-test.