Figure 1
Pathogenicity of M. oryzae ssd1 mutant against barley. (A) Pathogenicity assay by spray inoculation of the wild-type strain Hoku-1, and mossd1 mutants K1 and K4. Conidial suspension (1 × 106 conidia/ml) was sprayed onto barley leaves and incubated at 24°C. Typical blast lesions were observed on the inoculated leaves with Hoku-1 but not K1 and K4. Photographs were taken 5 days post inoculation. (B) Pathogenicity assay by droplet inoculation of the wild-type Hoku-1 and mossd1 mutant K1. Conidial suspensions (1 × 105 conidia/ml) were spotted onto leaf blades and incubated at 24°C. On intact leaves, severe blast lesions were observed at sites inoculated with Hoku-1, but not K1. On wounded leaves, brown deposition were observed at inoculated sites with both Hoku-1 and K1 but spreading of the lesions only occurred with Hoku-1.