Figure 3

Arrest of shoot and root growth by constitutive FAF expression. (A) Arrested shoot meristem in a strong 35S::FAF3 seedling. Expression of the other FAF genes by the 35 S promoter caused similar phenotypes (data not shown) (B) Close-up of arrested seedling under the SEM. (C-G) Root development of wild-type control (C) and intermediate 35S::FAF1 (D), 35S::FAF2 (E), 35S::FAF3 (F), and 35S::FAF4 (G) plants. The growth of the primary root is inhibited and the formation of adventitious roots is induced by high levels of FAF expression (D-G). (H) Rescue of root growth of a 35S::FAF3 line by exogenous sucrose (1%). (I) Quantification of the effect of sucrose on root growth in Col-0 and 35S::FAF plants (n = 20). Scale bars: 0.5 mm (A), 200 μm (B), 5 mm (C), 2 mm (D-G), 1 cm. (H).