Figure 5

WRKY18- and WRKY40-dependency of ABA-induced expression of WRKY60. Three-weeks-old wild-type and mutant plants were sprayed with water (Mock) or 5 μM ABA. Leaves from four treated plants were harvested at indicated times after the treatment for isolation of total RNA and analysis of WRKY18 (A), WRKY40 (B) and WRKY60 (C) transcripts using qRT-PCR. Expression level was defined as the ratio of qRT-PCR result of treated sample to its respective mock. The means and standard errors were calculated from three independent experiments. Asterisks mark statistically significant differences of expression level between ABA-treated-leaves harvested immediately and after indicated time. (Asterisks: p-value < 0.05; Double Asterisks: p-value < 0.01; by Student-Newman-Keuls Test).