Figure 1

Binding and transcriptional activities of CEBFs. (A) Binding of CEBFs to the CE1 element. The binding activity of six CEBFs was confirmed in yeast. Reporter yeast containing the lacZ reporter gene with (CE1) or without (-) the CE1 element was transformed with DNA from positive clones, and the transformants were grown in the glucose- or galactose-containing medium and assayed for the β-galactosidase activity by filter lift assay. (B) Transcriptional activity of CEBFs. CEBFs were cloned in frame with LexA DB, the fusion constructs were introduced into reporter yeast containing the lacZ reporter, and the reporter activity was measured by a liquid β-galactosidase assay. The numbers indicate the enzyme activity in Miller units. Each data point represents the mean of four independent measurements, and the small bars indicate the standard errors.