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Table 2 Properties and motifs of maize SUN-domain protiens.

From: Structure and expression of the maize (Zea maysL.) SUN-domain protein gene family: evidence for the existence of two divergent classes of SUN proteins in plants

 

Predicted propertiesa

Motifse

Class

Name

Lengthb

kDa

pIc

Cysd

TMf

SUNg

CCh

PADi

CCSD

ZmSUN1

462

51

9.1

3

W116-W141

N315-K454, (6 e-39)

F165-D228

 
 

ZmSUN2

439

48

7.8

3

T84-W109

P294-G425 (3 e-32

D166-L192

 

PM3

ZmSUN3

613

68

4.9

7

TM1, L33-V55

TM2, L555-M577

TM3, L599-I612

F233-D357 (2 e-38)

A482-F515

G437-G474

 

ZmSUN4

639

71

5.2

9

TM1, G58-L75

TM2, L581-M603

TM3, G621-I638

F257-D381 (7 e-38)

D514-E539

G463-G500

 

ZmSUN5

589

64

5.3

9

TM1, V46-L66

TM2, L525-C544

TM3, M572-Y588

H197-D321 (9 e-35)

CC1, V414-E434 CC2, K495-K523

G407-G444

  1. aProtein ORFs used were predicted from the sequences listed under cDNA from Table 1. Properties were calculated by means of the online ProtParam software, http://us.expasy.org/tools/protparam.html[80].
  2. bTotal number of amino acids in the predicted ORF.
  3. cpI, predicted isoelectric point.
  4. dTotal number of cysteine residues.
  5. eMotifs and domains are indicated by the first and last amino acid; the amino acid numbers for the ORFs are those from the sequences listed under cDNA from Table 1.
  6. fTM, locations of transmembrane regions predicted by the online software www.ch.embnet.org/software/TMPRED_form.html[70]. The multiple TMs of the PM3 proteins are named TM1, TM2, and TM3 according to the order of their occurrence starting from the N-terminus.
  7. gSUN, Sad1_UNC superfamily (pfam07738) domain locations and significance values are from alignments to the Conserved Domain Database (CDD version 2.21, NCBI), http://www.ncbi.nlm.nih.gov/Structure/cdd/cdd.shtml[81].
  8. hCC, coiled-coil motifs are predicted from the online COILS software http://www.ch.embnet.org/software/COILS_form.html[69]. The two CCs in SUN5 are called CC1 and CC2 according to the order of their occurrence starting from the N-terminus.
  9. iPAD, PM3-associated domain of unknown function defined here by multiple sequence alignments.