Figure 2From: Proteomic characterization of iron deficiency responses in Cucumis sativusL. roots2-DE maps. 2-DE maps of soluble protein fractions extracted from roots of cucumber plants grown for 5 and 8 d in the presence (+Fe) or absence (-Fe) of Fe. Proteins (400 μg per gel) were separated by IEF at pH 4-7, followed by 10% SDS PAGE and visualized by cCBB-staining. The number of spots detected was 2029 ± 272 for -Fe 5 d, 2136 ± 330 for +Fe 5 d, 1999 ± 223 for -Fe 5 d and 2208 ± 168 for +Fe 8 d.Back to article page