Figure 4
From: A high-throughput method for the detection of homoeologous gene deletions in hexaploid wheat
Amplification profiles of TaPFT1 homoeologues in Chinese Spring nullisomic tetrasomic lines. Fluorescent probe assay on 'wild type' Chinese Spring (A) and samples of Chinese Spring nullisomic tetrasomic wheat lines known to be deficient for chromosomes 5A(B), 5B(C) and 5D(D). A: Reaction features strong, efficient fluorescence from all probes. B: Reaction features strong efficient fluorescence from FAM-labelled (TaPFT1-B specific), and NED-labelled (TaPFT1-D specific) probes, but very inefficient fluorescence from the VIC-labelled (TaPFT1-A specific) probe. C: Reaction features strong efficient fluorescence from VIC-labelled (TaPFT1-A specific), and NED-labelled (TaPFT1-D specific) probes, but very inefficient fluorescence from the FAM-labelled (TaPFT1-B specific) probe. D: Reaction features strong efficient fluorescence from VIC-labelled (TaPFT1-A specific), and FAM-labelled (TaPFT1-B specific) probes, but very inefficient fluorescence from the NED-labelled (TaPFT1-D specific) probe.