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Table 1 Distinct sugarcane pri-miRNAs identified in this study

From: Identification and expression analysis of microRNAs and targets in the biofuel crop sugarcane

miRNA

Sequence source

Sequence ID

Sugarcane MIRgene

miRNA Mature Sequence

Location

NM (nt)

LP (nt)

MFEI

Conserved in rice

miR156a

EST

TC110664

SsMIR156b/c

UGACAGAAGAGAGUGAGCAC

5'

0(0)

411

0.81

Yes

miR159a

EST

TC79108

SsMIR159

UUUGGAUUGAAGGGAGCUCUG

3'

0(0)

265

0.78

Yes

miR167a

EST

TC105794

SsMIR167

UGAAGCUGCCAGCAUGAUCUG

5'

0(0)

193

0.93

Yes

miR168a

EST

TC97302

SsMIR168

UCGCUUGGUGCAGAUCGGGAC

5'

0(0)

104

0.86

Yes

miR169

EST

TC105581

SsMIR169

UAGCCAAGGAUGACUUGCCGG

5'

0(1)

148

0.92

Yes

miR396a

EST

CA240723

SsMIR396

UUCCACAGCUUUCUUGAACUG

5'

0(0)

134

1.02

Yes

miR827

EST

CA215078

SsMIR827

UUAGAUGACCAUCAGCAAACA

3'

0(1)

140

1.01

Yes

miR408a

EST

TC108481

SsMIR408a

CUGCACUGCCUCUUCCCUGGC

3'

0(1)

215

0.67

Yes

 

EST

TC74315

SsMIR408b

CUGCACUGCCUCUUCCCUGGC

3'

0(1)

283

0.80

Yes

miR437

EST

CA185316c

SsMIR437a

AAAGUUAGAGAAGUUUGACUU

3'

0

195

1.36

Yes

 

EST

CA191146c

SsMIR437b

AAAGUUAGACAAGUUUGACAU

3'

2

233

0.92

Yes

 

EST

CA300436c

SsMIR437c

AAAGUUAGAGAAGUCUGACUU

3'

1

197

1.36

Yes

miR444

EST

CA186150

SsMIR444a

UGCAGUUGUUGCCUCAAGCUU

3'

0

105

1.31

Yes

 

EST

CA105916

SsMIR444b

UGCAGUUGUUGCCUCAAGCUU/UUGUGGCUUUCUUGCAAGUUG

3'

0

132

1.24

Yes

 

EST

TC110432

SsMIR444c

UGCAGUUGUUGUCUCAAGCUU/UGUUGUCUCAAGCUUGCUGCC

3'

0

152

1.20

Yes

miR528

EST

CA290495

SsMIR528

UGGAAGGGGCAUGCAGAGGAG

5'

0

94

0.86

Yes

miR1128

EST

CA222833c

SsMIR1128

UACUACUCCCUCCGUCCCAAA

5'

1

275

1.17

No

miR1432

BAC

FJ348731

SsMIR1432

CUCAGGAAAGAUGACACCGAC

5'

1

118

1.15

Yes

miR319b

EST

TC87836

SsMIR319

UUGGACUGAAGGGUGCUCCC

3'

0(0)

n.d.

n.d.

Yes

  1. aSugarcane miRNA families deposited in the miRbase v. 14.
  2. bPri-miRNA identified only by precursor sequence homology. Therefore, we could assign neither LP nor MFEI values to this precursor.
  3. NM, nucleotide mismatches with rice, sorghum and Arabidopsis (in parentheses), when applicable.
  4. LP, length of the pre-miRNA.
  5. MFEI, minimal free energy index.
  6. CSugarcane pri-miRNAs with similarity (evalue <e-10) to MITE-derived hairpin sequences. miR437 precursors have similarity with Oryza sativa MITE-adh type A (2.5e-17) while miR1128 precursor is similar to O. glumipatula pangrangja MITE element (1.2e-11).