Figure 1

Elemental analysis of mature seeds from P. vulgaris and P. coccineus using micro-PIXE. Elemental maps obtained by Dynamic Analysis method. Maps of iron (a-e) and phosphorus (p-t). Colour scales showing concentrations are on the left side. f to j show the analyzed tissue, and the scanned area is marked with an open square. The maps a,b,p, and q represent the iron (Fe) and phosphorus (P) distribution in the cotyledon (cot) and radicle (rad) of P. vulgaris accession G14519 shown in f and g, respectively. The scanning of a region (shown in h) of the cotyledon of the P. vulgaris genotype G4825 is represented in the maps c and r. The analysis of the cotyledon and radicle of P. coccineus shown in i and j resulted in the maps d,e,s, and t. The maps k to o show the regions used for the quantification of elements displayed in Table 2. Each region has been assigned a unique number. Seed coat: sc. The * is used to mark an area that has been contaminated, and therefore elements cannot be quantified in the region. Scale bars: 0.5 mm.