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Table 1 Distribution of microsatellites in the unigene sequences of sugarcane

From: Functionally relevant microsatellites in sugarcane unigenes

Characters under study

Unigenes*

Number of sequences examined

15,594

Size (bp) of examined sequences

9,17,43,95

Number of identified perfect microsatellites

2,712 (17.4)

Number of perfect microsatellite containing sequences

2,230 (14.3)

Number of perfect microsatellite (excluding mononucleotides) containing sequences

584 (3.7)

Number of sequences containing more than one perfect microsatellites

167 (28.6)

Number of sequences containing single and unique perfect microsatellites

417 (71.4)

Number of mononucleotides

1,871 (12)

Number of dinucleotides

200 (23.8)

Number of trinucleotides

615 (73.1)

Number of tetranucleotides

15 (1.8)

Number of pentanucleotides

7 (0.83)

Number of hexanucleotides

4 (0.47)

Number of perfect microsatellites excluding mononucleotides

841 (5.4)

Size (kb) of sequences containing one perfect microsatellite

10.9

Number of perfect class I microsatellites

207 (24.6)

Size (kb) of sequences containing one perfect class I microsatellite

44.3

Number of primer pairs designed for perfecta microsatellites

810 (96.3)

Number of compound class I microsatellite containing sequences

183

Size (kb) of sequences containing one compound class I microsatellite

50.1

Number of compound class I microsatellites

183 (1.2)

Number of compound interrupting class I microsatellites

137 (74.9)

Number of compound non-interrupting class I microsatellites

46 (25.1)

Number of primer pairs designed for compound class I microsatellites

151 (82.5)

  1. *The number in the bracket is the proportion expressed in percentage
  2. aMononucleotides to hexanucleotides repeated up to 100 times without any interruption at a locus