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Table 4 Differences between Sanger- and 454-sequences of BAC Cm13_J04.

From: Sequencing of 6.7 Mb of the melon genome using a BAC pooling strategy

Length of Sanger-sequence

98,716 bp

   

Stretches of Ns on 454-sequence

5

3,572 bp (3.6%)

  

Homopolymers

length

Sanger 1

454 differences 2

   

No.

Motif

A/T

    
 

≤10

847

0

 
 

11

5

0

 
 

12

5

3

(A/T)11

 

13

3

2

(A/T)12

 

14

2

1

(A/T)13

 

15

3

2

(A/T)14

   

1

(A/T)13

 

16

1

1

(A/T)14

 

17

3

2

(A/T)15

 

18

1

1

(A/T)17

 

22

1

1

(A/T)19

 

24

1

0

 
 

28

1

1

A15CA13

C/G

    
 

5-7

49

0

 

Other

Sanger

454

 

(CT)15

(CT)15CTACTTACTTACTTACNNNNNNNC(CT)14

 

(GA)21

(GA)21GTAGTACGTACN23(GA)6

  1. 1Number of homopolymers in the Sanger sequence
  2. 2Number of homopolymers in the 454 sequence showing differences with the corresponding homopolymers in the Sanger sequence