Figure 7
SDS-PAGE and immunoblotting analysis. Maize kernels were germinated in water (c, control) or were treated with smoke (s, 1:1000 dilution) or KAR1 (KAR1, 0.1 μM) for 3, 4.5, 6 and 7.5 h and proteins were extracted from the embryos (n = 15). The experiments were repeated three times with similar results. Molecular masses (kDa) of standard proteins are indicated on the left. A, Immunoblotting analysis with anti-ubiquitin antibody. B, Protein pattern obtained by SDS-PAGE and Coomassie Blue staining.