Figure 4

Validation of microarray results via quantitative real-time PCR. A, Quantitative real-time PCR analysis was performed for 14 genes under the same conditions and design used for microarray analysis. Real-time PCR data were obtained from three independent experiments with similar results, and four amplification reactions. Microarray data (least-square means) were plotted against data from qRT-PCR and fitted into a linear regression. Both x- and y-axes are shown in log2 scale. B, The biological variation of the expression (assessed by quantitative real-time PCR analysis) of 8 selected master genes are shown. Experimental design is as for Fig. 5A. Error bars represent standard deviation. Asterisks indicate significant difference (p < 0.05) from the control samples (statistical analysis was assessed by a t-test).