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Table 1 Experimental Functional Annotation of VvTPS Genes of the VvTPS-a Subfamily

From: Functional Annotation, Genome Organization and Phylogeny of the Grapevine (Vitis vinifera) Terpene Synthase Gene Family Based on Genome Assembly, FLcDNA Cloning, and Enzyme Assays

Functional Gene ID

Function

Access. #

FLcDNA ID

Nearest VvTPS gene model

Representative Products

%

VvGwECar1

(E)-β-Caryophyllene Syn

HM807373

Gw38F6*

VvTPS01

(E)-caryophyllene

71

VvGwECar2

 

HM807374

Gw53B1*

VvTPS27

α-humulene

23

VvGwECar3

 

HM807375

Gw12001M3*

VvTPS02

germacrene D

6

VvPNECar1

 

HM807402

CAN82172^

VvTPS02

  

VvPNECar2

 

HM807403

CAO16256^

VvTPS13

  

VvGwGerA

Germacrene A Syn

HQ326230

Gw38F3*

VvTPS01

germacrene A

52

     

α-selinene

24

     

selin-11-en-4-α-ol

12

VvGwaBer

(E)-α-Bergamotene Syn

HM807376

Gw56B1*

VvTPS10

(E)-α-bergamotene

56

     

Unknown

17

     

Nerolidol

14

     

(E)-β-farnesene

8

     

(Z)-α-farnesene

5

VvGwGerD

Germacrene D Syn

HM807377

Gw64B1*

VvTPS07

germacrene D

94

VvPNGerD

 

HM807378

PN39M3*

VvTPS15

germacrene B

6

VvCSaFar

(E,E)-α-Farnesene syn

HM807379

CS102B7*

VvTPS20

(E,E)-α-farnesene

100

VvGwgCad

γ-Cadinene Syn

HM807380

Gw330M5*

VvTPS08

γ-cadinene

83

     

Unknown

17

VvPNbCur

β-Curcumene syn

HM807381

PN62M1*

VvTPS30

β-curcumene

22

     

(E)- γ-bisabolene

18

     

Iso-italicene

16

     

(-)-α-bisabolol

14

     

Î’-bisabolene

7

     

epi-β-santalene

7

     

unknown

5

     

(E)-β-farnesene

3

     

γ-curcumene

3

     

unknown

2

     

(Z)-α-bergomotene

1

     

unknown

1

VvPNSesq

Sesquithujene Syn

HM807404

CAO16252^

VvTPS12

(E)-α-bergomotene

1

     

sesquithujene

80

     

(E)-α-bergamotene

4

     

sesquisabinene

8

     

β-bisabolene

4

     

γ-bisabolene

2

     

unknown pm 222

1.5

     

β-bisabolol

0.5

VvPNaZin

α-Zingiberene syn

HM807405

CAO16257^

VvTPS14

α-zingiberene

79.5

     

β-sesquiphellandrene

17.5

     

β-bisabolene

3

VvPNSeInt

Selina-411-diene/Interme deol syn

HM807406

CAO39293^

VvTPS24

selina-4,11-diene

34

     

intermedeol

30

     

7-epi-α-selinene

15

     

δ-selinene

14

     

α-guaiene

3.5

     

selina-511-diene

2

     

unknown pm 204

1

VvPNCuCad

Cubebol/δ-Cadinene syn

HM807407

CAN76781^

VvTPS26

cubebol

20.5

     

δ-cadinene

20

     

unknown pm 222

16.5

     

α-cubebene

14

     

α-copaene

13.5

     

α-gurjunene

7.5

     

γ-cadinene

3

     

β-cubebene

2.5

     

unknown pm 204

2.5

VvPNaHum

α-Humulene syn

HM807408

CAN64791^

VvTPS11

α-humulene

56

     

hyemalol

37.5

     

(E)-β-caryophyllene

6.5

VvPNEb2epi Car

(E)-β-Caryophyllene/2-epi-(E)-β-Caryophyllene

HM807409

CAO39418^

VvTPS21

(E)-β-caryophyllene

72.5

     

2-epi-(E)-β-caryophyllene

25

  1. Terpenoids produced by individual VvTPS clones when incubated with FPP are listed. FLcDNA clones with redundant functions are listed as well. Clones marked with an * were characterized by in vitro assays with isolated recombinant VvTPS; clones marked with ^ were characterized in vivo in metabolically engineered E. coli. The tissue specific cDNA used to clone a particular gene is indicated in FLcDNA ID with either B (berry), F (flower), or M (mixed template consisting of stems, berries, flowers, and leaves) when appropriate. FLcDNA ID in bold indicates clone came from cDNA of methyl jasmonate treated tissue. Of the clones labelled as (E)-β-caryophyllene syn all produced an abundance of (E)-β-caryophyllene, with minor components of α-humulene and germacrene d except CAN82172 which did not produce germacrene D. Bolded VvTPS gene models indicate proof of transcript not previously known from the available ESTs.
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BMC Plant Biology

ISSN: 1471-2229

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