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Table 2 Fatty acid profiles for different homozygous FAD2-1 genotypes in four segregating populations.

From: Mutant alleles of FAD2-1A and FAD2-1Bcombine to produce soybeans with the high oleic acid seed oil trait

Fatty Acid
  16:0 18:0 18:1 18:2 18:3
Population 1 (Jake1 × PI 283327)
BB2 (n = 24) 12.2 ± 0.9 3.9 ± 0.5 20.5 ± 2.6 53.4 ± 2.8 10.0 ± 0.3
bb (n = 30) 11.2 ± 0.7 3.8 ± 0.6 29.4 ± 6.0 47.0 ± 5.1 8.7 ± 0.5
Population 2 (M23 × PI283327)
AABB (n = 5) 12.3 ± 0.5 3.7 ± 0.4 19.9 ± 3.3 55.4 ± 2.7 8.7 ± 1.0
AAbb (n = 5) 11.0 ± 0.5 3.9 ± 0.4 30.8 ± 5.2 45.9 ± 4.6 8.5 ± 0.9
aaBB (n = 14) 10.8 ± 0.8 3.8 ± 0.6 39.4 ± 5.7 37.1 ± 4.8 8.9 ± 1.2
aabb (n = 16) 7.9 ± 0.7 3.7 ± 0.6 82.2 ± 1.2 2.3 ± 0.6 3.9 ± 0.5
Population 3 (M23 × PI 567189A)
AABB (n = 11) 12.5 ± 0.9 2.9 ± 0.4 26.3 ± 7.4 51.4 ± 6.4 6.1 ± 1.2
AAbb (n = 3) 12.4 ± 0.8 2.8 ± 0.4 31.1 ± 4.5 47.5 ± 3.3 6.1 ± 1.0
aaBB (n = 1) 10.3 ± 0.6 2.8 ± 0.3 48.2 ± 7.2 32.5 ± 6.1 6.2 ± 0.9
aabb (n = 16) 8.4 ± 0.8 2.6 ± 0.4 80.0 ± 4.0 5.0 ± 3.0 3.8 ± 0.6
Population 4 F2(17D × S08-14788)
AABB (n = 5) 12.3 ± 0.9 3.2 ± 0.3 20.1 ± 0.9 55.7 ± 1.0 8.7 ± 0.6
AAbb (n = 5) 12.1 ± 1.0 3.4 ± 0.5 26.5 ± 4.5 47.8 ± 3.7 10.2 ± 0.9
aaBB (n = 6) 11.7 ± 0.3 3.0 ± 0.2 26.8 ± 1.4 48.2 ± 0.7 9.9 ± 0.5
aabb (n = 12) 7.8 ± 0.5 3.1 ± 0.2 81.1 ± 2.2 3.2 ± 1.4 4.9 ± 0.6
Population 4 F2:3 (17D × S08-14788)
AABB (n = 5) 9.6 ± 0.6 3.9 ± 0.4 22.4 ± 2.9 56.0 ± 2.8 8.2 ± 0.9
AAbb (n = 4) 10.5 ± 0.5 3.8 ± 0.3 23.1 ± 2.5 54.0 ± 2.6 8.6 ± 0.5
aaBB (n = 6) 9.3 ± 0.6 3.2 ± 0.3 35.0 ± 7.8 42.9 ± 5.9 9.6 ± 2.2
aabb (n = 11) 6.9 ± 0.4 3.2 ± 0.2 77.3 ± 2.0 6.3 ± 1.5 6.3 ± 0.6
  1. 1Jake is a soybean line with normal oleic acid content and wild-type FAD2-1A; M23 and 17D are soybean lines with mutant FAD2-1AΔ and FAD2-1A S117N alleles, respectively. PI 283327 and PI 567189A are soybean lines with mutant FAD2-1B P137R and FAD2-1B I143T alleles, respectively. S08-14788 is a soybean line selected from population 2 that inherited the mutant FAD2-1B P137R alleles from PI 283327. 2AA = wild-type FAD2-1A alleles, aa = mutant FAD2-1A alleles derived from M23 or 17D, BB = wild-type FAD2-1B alleles, bb = mutant FAD2-1B alleles derived from PI 283327 or PI 567189A.