Figure 5
Expression patterns of flavonoid biosynthetic pathway genes in GLRaV-3-infected symptomatic and virus-free green leaves. The relative expression levels of (a) the flavonoid biosynthetic pathway genes and (b) the MybA1 gene in GLRaV-3-infected (designated as virus-infected) and virus-free leaves are shown as arbitrary units on the y-axis. The raw Cq values for each gene was normalized using two reference genes (NF[Actin and NAD5]). Columns represent mean value from five biological replicates, except in case of MybA1 that represents only four biological replicates and vertical bars indicate standard errors. Significant differences between virus-infected and virus-free leaves was determined by one-way ANOVA, using the SigmaPlot 11 software and indicated by asterisks (* = p < 0.05 and ** = p < 0.001). See legend for Figure 1 for names of genes.