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Table 1 Amplification procedure of AmCBL1 promoter

From: Identification and functional characterisation of the promoter of the calcium sensor gene CBL1 from the xerophyte Ammopiptanthus mongolicus

Step procedure primers template
First intron amplification INT1/INT2 genome DNA
First walking linear amplification reaction add poly C to end GSP1 genome DNA
  amplification of poly-added production GSP2/AAP poly-added production
  amplification of target gene GSP3/AUAP last step production
PCR amplification W1/GSP2 genome DNA
second walking linear amplification reaction add poly C to end GSP4 genome DNA
  amplification of poly-added production GSP5/AAP poly-added production
  amplification of target gene GSP6/AUAP last step production
PCR amplification W1/GSP2 genome DNA
third walking linear amplification reaction add poly C to end GSP7 genome DNA
  amplification of poly-added production GSP8/AAP poly-added production
  amplification of target gene GSP9/AUAP last step production
AmCBL1 promoter amplification S1/GSP2 genome DNA