Dot-blot for DNA methylation in maize root tip cells treated with TSA or 5-AC. (A) Dot-blot analysis shows that 5-meC decreased in maize root tips after treatment with either 5-AC or TSA. (B) After immunodetection, the same membrane was stained with ethidium bromide to confirm equal DNA loading. (C) Mean gray values of dot-blots shown in (A) and (B). The mean gray value for 5-meC following treatment with either TSA or 5-AC was significantly lower than that of the control, while the mean gray value for DNA content in the control was not significantly different from that of the TSA- or 5-AC-treated sample. Error bars represent standard error of the mean.