Complementation tests of transgenic plants. (A) A cDNAs of Pish(t) under the control of the 35 S promoter was introduced into KM. Control plants were transformed with the empty vector (Vec). Three-week-old plants of the T1 and T2 generations were challenged with a rice blast isolate containing avrPish by spray inoculation. The photographs show typical phenotypes of leaf blades 7 days post inoculation. Results were reproduced in at least three independent infection experiments using five or more plants each of three independent transgenic lines. (B) RT-PCR analysis was performed on total RNA isolated from leaves of T1 plants transformed with the empty vector or Pish(t). An Action gene was used as a control for RNA template amounts.