YAO is localized in the nucleolus. (A) Confocal micropgraphs showing nuclear localization of YAO-GFP in root tip. (B-D) Confocal micrographs of the same cell showing the subcellular localization of YAO-GFP (B), nucleolar marker SWA2-DsRed (C), and the merged image (D), note the nucleolar localization of YAO. (E-H) Confocal micrographs showing YAO-GFP localization in megaspore mother cell (E), embryo sac and integument cells (F), zygote and endosperm (G), endosperm and preglobular embryo (H) nuclei. ac, apical cell; ap, antipodal cell; bc, basal cell. e, endosperm; ec, egg cell; ms, megaspore mother cell; pn, polar nucleus; sg, synergid; z, zygote. Bar = 20 μm.