Figure 4
Growth performance and transcript abundance of OsIRO3 in over-expressing (OE) lines. Three week old transgenic and vector only transformed rice plants, the latter referred to as wild-type, were transferred to solution culture containing 100, 10, or 1 μM EDTA-Fe for 10 days. (A) Expression levels of OsIRO3 in OE plants grown in normal solution culture compared to wild-type. The expression level of wild-type was normalized to one. (B) The youngest leaf of OsIRO3-OE and wild-type plants in solution culture with 100 μM, 10 μM, or 1 μM Fe-EDTA. (C) Shoot height of OsIRO3-OE and wild-type under the various Fe conditions. (D) SPAD values of newly expanded leaves of OsIRO3-OE and wild-type plants. (E) Shoot Fe concentrations of OsIRO3-OE lines and wild-type.