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Table 1 Segregation of flower color in F1 plants and F2 population derived from a cross between a soybean cultivar Harosoy with purple flowers and B09121, a Glycine soja accession with light purple flowers, and segregation of flower color in F1 plants, and F2 and F3 populations derived from a cross between a soybean near-isogenic line Clark-w1 with white flowers and B09121 in Tsukuba, Japan.

From: A new allele of flower color gene W1 encoding flavonoid 3'5'-hydroxylase is responsible for light purple flowers in wild soybean Glycine soja

Generation Year Number of plants Expected ratio χ2 value Probability
(Pvalue)
   Total Purple Light purple White    
B09121 (B) 2007 10 - 10 - - - -
Harosoy (H) 2007 10 10 - - - - -
H × B F1 2007 3 3 - - - - -
H × B F2 2007 119 91 28 - 3:1 0.14 0.71
Clark-w1 (w1) 2007 10 - - 10 - - -
w1 × B F1 2007 3 - 3 - - - -
w1 × B F2 2007 124 1 98 25 3:1a 1.43 0.23
w1 × B F3 (purple)b 2008 69 51 18 - 3:1 0.04 0.83
  1. aPlants with light purple flowers: plants with white flowers.
  2. bA line derived from an F2 plant with purple flowers.