Genotypes of parents (P) and offspring (F1) of the cross used to map diplospory in T. officinale. (A) Crossing scheme showing the sexual diploid maternal plant S2.125 (dd, dotted lines), the diplosporous tetraploid pollen donor PAX (Dddd, with D = solid line), and their possible triploid offspring (Ddd, ddd, and D/d-dd recombinants). S8 and S10 span DIP in 2.7 cM , and the S8+/S10+ genotypes represent the D-alleles. (B) Agarose gel showing amplicons of S8 (upper half) and S10 (lower half) of 14 F1 and the two parent plants, with the diagnostic products (black arrows) and recombinant plants (white arrows) indicated. Plants with both markers present were interpreted as being diplosporous and those with both markers absent as being meiotic. N = negative control and L = l00 bp ladder.