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Table 4 FAE1 polymorphisms between A and C genomes

From: Assessment of FAE1 polymorphisms in three Brassica species using EcoTILLING and their association with differences in seed erucic acid contents

No.

Positions

Nucleotide Differences Between A and C Genomes

Amino acid difference

Restriction Enzymes

1

63a

C Tb

C21=c

 

2

72

G A

P24=

AccI, HindII, HpaI, MjaIV, TspGWI

3

168

C T

I56=

BsrDI, BccI, BtgZI

4

312

T C

Y104=

 

5

363

G C

S121=

EcoRII, ScrFI, Hpy99I

6

417

T C

T139=

DsaI, SecI

7

531

C T

N177=

AclI, MaeII

8

591

A T

P197=

BccI, BtgZI

9

856

G A

G286R

Eco57MI, EcoRII, GsuI, ScrFI

10

1036

T C

L346=

BseYI, AclI, MaeII

11

1113

T C

D371=

 

12

1128

C T

Y376=

 

13

1140

T C

D380=

 

14

1146

A G

K382=

AluI, Cac8I, CviJI, HindIII

15

1155

T C

I385=

TaqI

16

1184

G A

R395K

 

17

1217

C G

A406G

AvrII, HaeIII, SecI, StuI, StyI

18

1464

G C

V488=

MseI, Hin4I

  1. a63 indicates the position 63 bp from the base 'A' in the start codon.
  2. bThe first base is in the A genome and the second is in the C genome.
  3. cC21 = indicates no change in the amino acid (synonymous variation)